microarray analysis suite instrument system (Thermo Fisher)
Structured Review

Microarray Analysis Suite Instrument System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microarray analysis suite instrument system/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
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1) Product Images from "Cigarette smoke condensate induces cytochromes P450 and aldo-keto reductases in oral cancer cells"
Article Title: Cigarette smoke condensate induces cytochromes P450 and aldo-keto reductases in oral cancer cells
Journal: Toxicology letters
doi: 10.1016/j.toxlet.2006.03.008
Figure Legend Snippet: Principal component analysis of microarray data to demonstrate similarity of response of the cell types and treatment times. In the plot, each point represents a cell line (NHEK, Leuk1, Leuk2, or 101A) and treatment time (0 h, black circles; 24 h, gray triangles) of CSC exposure (25 μg/ml). The X, Y and Z-axes are PC#1, PC#2 and PC#3, respectively.
Techniques Used: Microarray
Figure Legend Snippet: Comparative quantitative real-time RT-PCR analysis. RNA from control (0 h) and treated (24 h, 25 μg/ml CSC) cells were subjected to quantitative real-time RT-PCR analysis using primers specific for CYP1A1, CYP1B1, AKR1C1, AKR1C3, AKR1B10, and the internal standard β-actin. (A) NHEK; (B) Leuk1; (C) Leuk2; (D) 101A cells; open bars, microarray data; solid bars, RT-PCR data. The fold change levels on the Y-axis are relative to the expression in untreated cells. Results are expressed as the mean and range of duplicate determinations for microarray data, and as mean ± S.D. of four independent experiments for qRT-PCR data: *p < 0.05; **p = 0.01; #non-significant.
Techniques Used: Quantitative RT-PCR, Microarray, Reverse Transcription Polymerase Chain Reaction, Expressing
Figure Legend Snippet: CSC-induced expression increases for selected target genes. (A) Basal expression levels in untreated cell lines NHEK, Leuk1, Leuk2, and 101A for the indicated target genes, as measured by microarray-derived target intensities. (B) Time course of induction. For each cell line, target intensities after CSC treatment for 0, 5, and 24 h are plotted for the following genes: CYP1A1 (□); CYP1B1 (▲); AKR1C1 (▼); AKR1C3 (■); AKR1B10 (○).
Techniques Used: Expressing, Microarray, Derivative Assay